Please use this identifier to cite or link to this item: https://repositorio.icmbio.gov.br/handle/cecav/1158
Title: First molecular evidence of frogs as a food source for sand flies (Diptera: Phlebotominae) in Brazilian caves
Authors: Costa, J.C.R.
Santos, C.S.
Keywords: Psychodidae;Sciopemyia;Amphibians;PCR;Conservation Units
Issue Date: 14-Apr-2021
Abstract: Genus and species of phlebotomine sand flies have been recorded and described in caves in Brazil, but no study has provided the food source used by sand flies in these environments. Herein, we identified the blood source used by sand fly species in caves located at “Quadrilátero Ferrífero” (QF), Minas Gerais state. Specimens were manually collected near or on anurans inside ferruginous caves in Serra do Gandarela National Park and Serra do Rola Moça State Park. Males and females were placed in vials with 70% alcohol and stored at −10°C. Females engorged, after specific identification, had DNA extracted and followed for PCR amplification using specific primers. Sequencing was analyzed in the GenBank and Barcode of Life. A total of 198 specimens were collected (107 females and 91 males), all of them belonging to species Sciopemyia aff. microps (88.89%), Sciopemyia sordellii (10.61%), or Martinsmyia oliveirai (0.50%). When it comes to the females, 89 were S. aff. microps and 18 S. sordellii. Nineteen engorged females of S. aff. microps were analyzed and most of them (n=18) presented blood from Bokermannohyla martinsi and one contained blood from Scinax fuscovarius. The blood present in engorged females of S. sordellii (n=4) was from B. martinsi. Sciopemyia genus specimens are commonly found in collections carried out inside natural caves, but this was the first study to prove that females of this genus feed on cold-blooded animals in nature.
metadata.dc.source: Springer
metadata.dc.type: Artigo
metadata.dc.localofdeposit: https://link.springer.com/article/10.1007/s00436-021-07154-3#citeas
URI: https://repositorio.icmbio.gov.br/handle/cecav/1158
Appears in Collections:BIOLOGIA SUBTERRÂNEA

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